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wild type wt c57bl 6j mice  (Jackson Laboratory)
86
Jackson Laboratory wild type wt c57bl 6j mice
ROCK activity increases after cold exposure in a ROCK2-dependent manner (A) The mRNA levels of Rock isoforms in interscapular brown adipose tissue (iBAT), inguinal subcutaneous white adipose tissue (sWAT), and epididymal (epiWAT) from 8-week-old male wild-type <t>(WT)</t> <t>C57BL/6J</t> mice at room temperature (RT) ( n = 6 mice) or after exposure to 4 °C for 3 h ( n = 5 mice). (B) The ROCK activity was indicated by the ratio of phosphorylated to total myosin-binding subunit (MBS) of myosin phosphatase, a direct downstream target of ROCK. Western blotting and quantification of ROCK1, ROCK2, phosphorylated MBS (pMBS), and total MBS (tMBS) in iBAT, sWAT, and epiWAT ( n = 4 mice per time point) from male WT mice housed at 4 °C for 0, 1, 3, or 6 h (C and D) Western blotting and quantification of ROCK1, ROCK2, phosphorylated and total MBS in sWAT from male WT and Rock2 +/− mice housed at RT or 4 °C for 6 h ( n = 4 mice per genotype). (E) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in iBAT, sWAT, and epiWAT from male Adipoq-Cre +/− Rock2 +/+ (control) and Adipoq-Cre +/− Rock2 flox/flox (AdR2KO) mice housed at RT or 4 °C for 3 h ( n = 4 mice per group). (F) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in sWAT from male control and AdR2KO mice housed at RT or 4 °C for 3 days ( n = 4 mice per group). The immunoblot images are representative of at least three independent experiments. p values were determined by two-tailed Student’s t test (A, C, and F) or one-way ANOVA with Tukey’s multiple comparisons test (B, D, E, and F). All data are presented as the mean ± SEM of at least three independent biological replicates.
Wild Type Wt C57bl 6j Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sex matched wild type wt c57bl 6 mice  (Jackson Laboratory)
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Jackson Laboratory sex matched wild type wt c57bl 6 mice
ROCK activity increases after cold exposure in a ROCK2-dependent manner (A) The mRNA levels of Rock isoforms in interscapular brown adipose tissue (iBAT), inguinal subcutaneous white adipose tissue (sWAT), and epididymal (epiWAT) from 8-week-old male wild-type <t>(WT)</t> <t>C57BL/6J</t> mice at room temperature (RT) ( n = 6 mice) or after exposure to 4 °C for 3 h ( n = 5 mice). (B) The ROCK activity was indicated by the ratio of phosphorylated to total myosin-binding subunit (MBS) of myosin phosphatase, a direct downstream target of ROCK. Western blotting and quantification of ROCK1, ROCK2, phosphorylated MBS (pMBS), and total MBS (tMBS) in iBAT, sWAT, and epiWAT ( n = 4 mice per time point) from male WT mice housed at 4 °C for 0, 1, 3, or 6 h (C and D) Western blotting and quantification of ROCK1, ROCK2, phosphorylated and total MBS in sWAT from male WT and Rock2 +/− mice housed at RT or 4 °C for 6 h ( n = 4 mice per genotype). (E) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in iBAT, sWAT, and epiWAT from male Adipoq-Cre +/− Rock2 +/+ (control) and Adipoq-Cre +/− Rock2 flox/flox (AdR2KO) mice housed at RT or 4 °C for 3 h ( n = 4 mice per group). (F) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in sWAT from male control and AdR2KO mice housed at RT or 4 °C for 3 days ( n = 4 mice per group). The immunoblot images are representative of at least three independent experiments. p values were determined by two-tailed Student’s t test (A, C, and F) or one-way ANOVA with Tukey’s multiple comparisons test (B, D, E, and F). All data are presented as the mean ± SEM of at least three independent biological replicates.
Sex Matched Wild Type Wt C57bl 6 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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male c57bl 6j wild type wt mice  (Jackson Laboratory)
86
Jackson Laboratory male c57bl 6j wild type wt mice
ROCK activity increases after cold exposure in a ROCK2-dependent manner (A) The mRNA levels of Rock isoforms in interscapular brown adipose tissue (iBAT), inguinal subcutaneous white adipose tissue (sWAT), and epididymal (epiWAT) from 8-week-old male wild-type <t>(WT)</t> <t>C57BL/6J</t> mice at room temperature (RT) ( n = 6 mice) or after exposure to 4 °C for 3 h ( n = 5 mice). (B) The ROCK activity was indicated by the ratio of phosphorylated to total myosin-binding subunit (MBS) of myosin phosphatase, a direct downstream target of ROCK. Western blotting and quantification of ROCK1, ROCK2, phosphorylated MBS (pMBS), and total MBS (tMBS) in iBAT, sWAT, and epiWAT ( n = 4 mice per time point) from male WT mice housed at 4 °C for 0, 1, 3, or 6 h (C and D) Western blotting and quantification of ROCK1, ROCK2, phosphorylated and total MBS in sWAT from male WT and Rock2 +/− mice housed at RT or 4 °C for 6 h ( n = 4 mice per genotype). (E) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in iBAT, sWAT, and epiWAT from male Adipoq-Cre +/− Rock2 +/+ (control) and Adipoq-Cre +/− Rock2 flox/flox (AdR2KO) mice housed at RT or 4 °C for 3 h ( n = 4 mice per group). (F) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in sWAT from male control and AdR2KO mice housed at RT or 4 °C for 3 days ( n = 4 mice per group). The immunoblot images are representative of at least three independent experiments. p values were determined by two-tailed Student’s t test (A, C, and F) or one-way ANOVA with Tukey’s multiple comparisons test (B, D, E, and F). All data are presented as the mean ± SEM of at least three independent biological replicates.
Male C57bl 6j Wild Type Wt Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wild type wt c57bl 6 mice  (Huachuang Securities Co Ltd)
86
Huachuang Securities Co Ltd wild type wt c57bl 6 mice
ROCK activity increases after cold exposure in a ROCK2-dependent manner (A) The mRNA levels of Rock isoforms in interscapular brown adipose tissue (iBAT), inguinal subcutaneous white adipose tissue (sWAT), and epididymal (epiWAT) from 8-week-old male wild-type <t>(WT)</t> <t>C57BL/6J</t> mice at room temperature (RT) ( n = 6 mice) or after exposure to 4 °C for 3 h ( n = 5 mice). (B) The ROCK activity was indicated by the ratio of phosphorylated to total myosin-binding subunit (MBS) of myosin phosphatase, a direct downstream target of ROCK. Western blotting and quantification of ROCK1, ROCK2, phosphorylated MBS (pMBS), and total MBS (tMBS) in iBAT, sWAT, and epiWAT ( n = 4 mice per time point) from male WT mice housed at 4 °C for 0, 1, 3, or 6 h (C and D) Western blotting and quantification of ROCK1, ROCK2, phosphorylated and total MBS in sWAT from male WT and Rock2 +/− mice housed at RT or 4 °C for 6 h ( n = 4 mice per genotype). (E) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in iBAT, sWAT, and epiWAT from male Adipoq-Cre +/− Rock2 +/+ (control) and Adipoq-Cre +/− Rock2 flox/flox (AdR2KO) mice housed at RT or 4 °C for 3 h ( n = 4 mice per group). (F) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in sWAT from male control and AdR2KO mice housed at RT or 4 °C for 3 days ( n = 4 mice per group). The immunoblot images are representative of at least three independent experiments. p values were determined by two-tailed Student’s t test (A, C, and F) or one-way ANOVA with Tukey’s multiple comparisons test (B, D, E, and F). All data are presented as the mean ± SEM of at least three independent biological replicates.
Wild Type Wt C57bl 6 Mice, supplied by Huachuang Securities Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wild type wt c57bl 6 mice - by Bioz Stars, 2026-05
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wild type wt c57bl 6j  (Jackson Laboratory)
86
Jackson Laboratory wild type wt c57bl 6j
ROCK activity increases after cold exposure in a ROCK2-dependent manner (A) The mRNA levels of Rock isoforms in interscapular brown adipose tissue (iBAT), inguinal subcutaneous white adipose tissue (sWAT), and epididymal (epiWAT) from 8-week-old male wild-type <t>(WT)</t> <t>C57BL/6J</t> mice at room temperature (RT) ( n = 6 mice) or after exposure to 4 °C for 3 h ( n = 5 mice). (B) The ROCK activity was indicated by the ratio of phosphorylated to total myosin-binding subunit (MBS) of myosin phosphatase, a direct downstream target of ROCK. Western blotting and quantification of ROCK1, ROCK2, phosphorylated MBS (pMBS), and total MBS (tMBS) in iBAT, sWAT, and epiWAT ( n = 4 mice per time point) from male WT mice housed at 4 °C for 0, 1, 3, or 6 h (C and D) Western blotting and quantification of ROCK1, ROCK2, phosphorylated and total MBS in sWAT from male WT and Rock2 +/− mice housed at RT or 4 °C for 6 h ( n = 4 mice per genotype). (E) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in iBAT, sWAT, and epiWAT from male Adipoq-Cre +/− Rock2 +/+ (control) and Adipoq-Cre +/− Rock2 flox/flox (AdR2KO) mice housed at RT or 4 °C for 3 h ( n = 4 mice per group). (F) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in sWAT from male control and AdR2KO mice housed at RT or 4 °C for 3 days ( n = 4 mice per group). The immunoblot images are representative of at least three independent experiments. p values were determined by two-tailed Student’s t test (A, C, and F) or one-way ANOVA with Tukey’s multiple comparisons test (B, D, E, and F). All data are presented as the mean ± SEM of at least three independent biological replicates.
Wild Type Wt C57bl 6j, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wild type wt c57bl 6j/product/Jackson Laboratory
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female wild type wt c57bl 6 mice  (Jackson Laboratory)
86
Jackson Laboratory female wild type wt c57bl 6 mice
ROCK activity increases after cold exposure in a ROCK2-dependent manner (A) The mRNA levels of Rock isoforms in interscapular brown adipose tissue (iBAT), inguinal subcutaneous white adipose tissue (sWAT), and epididymal (epiWAT) from 8-week-old male wild-type <t>(WT)</t> <t>C57BL/6J</t> mice at room temperature (RT) ( n = 6 mice) or after exposure to 4 °C for 3 h ( n = 5 mice). (B) The ROCK activity was indicated by the ratio of phosphorylated to total myosin-binding subunit (MBS) of myosin phosphatase, a direct downstream target of ROCK. Western blotting and quantification of ROCK1, ROCK2, phosphorylated MBS (pMBS), and total MBS (tMBS) in iBAT, sWAT, and epiWAT ( n = 4 mice per time point) from male WT mice housed at 4 °C for 0, 1, 3, or 6 h (C and D) Western blotting and quantification of ROCK1, ROCK2, phosphorylated and total MBS in sWAT from male WT and Rock2 +/− mice housed at RT or 4 °C for 6 h ( n = 4 mice per genotype). (E) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in iBAT, sWAT, and epiWAT from male Adipoq-Cre +/− Rock2 +/+ (control) and Adipoq-Cre +/− Rock2 flox/flox (AdR2KO) mice housed at RT or 4 °C for 3 h ( n = 4 mice per group). (F) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in sWAT from male control and AdR2KO mice housed at RT or 4 °C for 3 days ( n = 4 mice per group). The immunoblot images are representative of at least three independent experiments. p values were determined by two-tailed Student’s t test (A, C, and F) or one-way ANOVA with Tukey’s multiple comparisons test (B, D, E, and F). All data are presented as the mean ± SEM of at least three independent biological replicates.
Female Wild Type Wt C57bl 6 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wild type human axl axl wt  (Addgene inc)
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Addgene inc wild type human axl axl wt
ROCK activity increases after cold exposure in a ROCK2-dependent manner (A) The mRNA levels of Rock isoforms in interscapular brown adipose tissue (iBAT), inguinal subcutaneous white adipose tissue (sWAT), and epididymal (epiWAT) from 8-week-old male wild-type <t>(WT)</t> <t>C57BL/6J</t> mice at room temperature (RT) ( n = 6 mice) or after exposure to 4 °C for 3 h ( n = 5 mice). (B) The ROCK activity was indicated by the ratio of phosphorylated to total myosin-binding subunit (MBS) of myosin phosphatase, a direct downstream target of ROCK. Western blotting and quantification of ROCK1, ROCK2, phosphorylated MBS (pMBS), and total MBS (tMBS) in iBAT, sWAT, and epiWAT ( n = 4 mice per time point) from male WT mice housed at 4 °C for 0, 1, 3, or 6 h (C and D) Western blotting and quantification of ROCK1, ROCK2, phosphorylated and total MBS in sWAT from male WT and Rock2 +/− mice housed at RT or 4 °C for 6 h ( n = 4 mice per genotype). (E) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in iBAT, sWAT, and epiWAT from male Adipoq-Cre +/− Rock2 +/+ (control) and Adipoq-Cre +/− Rock2 flox/flox (AdR2KO) mice housed at RT or 4 °C for 3 h ( n = 4 mice per group). (F) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in sWAT from male control and AdR2KO mice housed at RT or 4 °C for 3 days ( n = 4 mice per group). The immunoblot images are representative of at least three independent experiments. p values were determined by two-tailed Student’s t test (A, C, and F) or one-way ANOVA with Tukey’s multiple comparisons test (B, D, E, and F). All data are presented as the mean ± SEM of at least three independent biological replicates.
Wild Type Human Axl Axl Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ROCK activity increases after cold exposure in a ROCK2-dependent manner (A) The mRNA levels of Rock isoforms in interscapular brown adipose tissue (iBAT), inguinal subcutaneous white adipose tissue (sWAT), and epididymal (epiWAT) from 8-week-old male wild-type (WT) C57BL/6J mice at room temperature (RT) ( n = 6 mice) or after exposure to 4 °C for 3 h ( n = 5 mice). (B) The ROCK activity was indicated by the ratio of phosphorylated to total myosin-binding subunit (MBS) of myosin phosphatase, a direct downstream target of ROCK. Western blotting and quantification of ROCK1, ROCK2, phosphorylated MBS (pMBS), and total MBS (tMBS) in iBAT, sWAT, and epiWAT ( n = 4 mice per time point) from male WT mice housed at 4 °C for 0, 1, 3, or 6 h (C and D) Western blotting and quantification of ROCK1, ROCK2, phosphorylated and total MBS in sWAT from male WT and Rock2 +/− mice housed at RT or 4 °C for 6 h ( n = 4 mice per genotype). (E) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in iBAT, sWAT, and epiWAT from male Adipoq-Cre +/− Rock2 +/+ (control) and Adipoq-Cre +/− Rock2 flox/flox (AdR2KO) mice housed at RT or 4 °C for 3 h ( n = 4 mice per group). (F) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in sWAT from male control and AdR2KO mice housed at RT or 4 °C for 3 days ( n = 4 mice per group). The immunoblot images are representative of at least three independent experiments. p values were determined by two-tailed Student’s t test (A, C, and F) or one-way ANOVA with Tukey’s multiple comparisons test (B, D, E, and F). All data are presented as the mean ± SEM of at least three independent biological replicates.

Journal: iScience

Article Title: Induction of adipocyte thermogenic program by Rho-associated coiled-coil containing kinase 2

doi: 10.1016/j.isci.2026.115225

Figure Lengend Snippet: ROCK activity increases after cold exposure in a ROCK2-dependent manner (A) The mRNA levels of Rock isoforms in interscapular brown adipose tissue (iBAT), inguinal subcutaneous white adipose tissue (sWAT), and epididymal (epiWAT) from 8-week-old male wild-type (WT) C57BL/6J mice at room temperature (RT) ( n = 6 mice) or after exposure to 4 °C for 3 h ( n = 5 mice). (B) The ROCK activity was indicated by the ratio of phosphorylated to total myosin-binding subunit (MBS) of myosin phosphatase, a direct downstream target of ROCK. Western blotting and quantification of ROCK1, ROCK2, phosphorylated MBS (pMBS), and total MBS (tMBS) in iBAT, sWAT, and epiWAT ( n = 4 mice per time point) from male WT mice housed at 4 °C for 0, 1, 3, or 6 h (C and D) Western blotting and quantification of ROCK1, ROCK2, phosphorylated and total MBS in sWAT from male WT and Rock2 +/− mice housed at RT or 4 °C for 6 h ( n = 4 mice per genotype). (E) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in iBAT, sWAT, and epiWAT from male Adipoq-Cre +/− Rock2 +/+ (control) and Adipoq-Cre +/− Rock2 flox/flox (AdR2KO) mice housed at RT or 4 °C for 3 h ( n = 4 mice per group). (F) Western blotting and quantification of ROCK1, ROCK2, pMBS, and tMBS in sWAT from male control and AdR2KO mice housed at RT or 4 °C for 3 days ( n = 4 mice per group). The immunoblot images are representative of at least three independent experiments. p values were determined by two-tailed Student’s t test (A, C, and F) or one-way ANOVA with Tukey’s multiple comparisons test (B, D, E, and F). All data are presented as the mean ± SEM of at least three independent biological replicates.

Article Snippet: Wild-type (WT) C57BL/6J mice were purchased from The Jackson Laboratory (Bar Harbor, ME).

Techniques: Activity Assay, Binding Assay, Western Blot, Control, Two Tailed Test